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. 2011 May 2;286(25):22426–22440. doi: 10.1074/jbc.M110.149252

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© 2011 by The American Society for Biochemistry and Molecular Biology, Inc.

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FIGURE 7. — Effects of epoxomicin and chloroquine on p62/SQSTM1 and ubiquitin-protein aggregates. Double immunofluorescence staining of SK-N-SH cells control or treated with 25 nm epoxomicin or 100 μm chloroquine alone or combined (Epx + CQ) is shown. p62/SQSTM1 (p62) aggregates were visualized with the anti-p62/SQSTM1 antibody (red), and mono- and polyubiquitinated proteins were visualized with the anti-ubiquitin antibody (green). Enlarged merged (ubiquitin /p62) images of cells marked with an asterisk and treated under the different conditions are shown in the bottom panels. Scale bar, 20 μm. Arrows point to ubiquitin-protein aggregates.