FIGURE 9.
Comparison of the effects of the autophagy inhibitors 3-MA, CQ, NH4Cl, and bafilomycin A1 (Baf) with the proteasome inhibitor Epx on ubiquitinated (Ub) proteins, p62/SQSTM1, LC3-I, and LC3-II in wild type (WT) and Atg5−/− MEFs. Western blot analyses (8 or 10% gels) to detect ubiquitinated proteins (A), p62/SQSTM1 (p62, B), LC3-I and LC3-II (C), and actin (D, loading control) in total extracts (40 μg of protein/lane) of MEFs treated with each inhibitor for 24 h. Molecular mass markers in kDa are shown on the right. The protein levels were semi-quantified by densitometry (E). Data represent relative intensity obtained from at least four cell culture dishes per condition.