FIGURE 2.

AA rescues HRMVEC DNA synthesis, migration, and tube formation from inhibition by cPLA₂ down-regulation. A–C, HRMVECs that were transfected with scrambled or cPLA₂ siRNA (100 nm) and growth-arrested were treated with and without VEGF (40 ng/ml) in the presence and absence of exogenously added AA (10 μm), and DNA synthesis (A), migration (B), and tube formation (C) were measured as described under “Materials and Methods.” The bar graphs in A–C represent the quantitative analysis of three independent experiments. The values are presented as mean ± S.D. *, p < 0.01 versus scrambled (Scr) siRNA; **, p < 0.01 versus scrambled siRNA + VEGF; ***, p < 0.01 versus cPLA₂ siRNA + VEGF.