FIGURE 7.
Circular permutation in the CBM62 family. BLAST analysis of CtCBM62, corresponding to residues 739–885 of CtXyl5A, identified five proteins that displayed >42% sequence identity, which are identified by their GenBankTM accession numbers. ABR45148.1 is an example of a group of 40 proteins that display noncontiguous similarity to CtCBM62. Extensive co-linear identity to CtCBM62 is evident after ABR45148.1 has been subject to a circular permutation. This genetic event is indicated by the crossover lines, leading to the ligation of the N and C terminus of the encoded protein and cleavage at the interface of the red and yellow sequence, resulting in the generation of a new N and C terminus. The sequence alignment of CtCBM62 (ABN53395) and ABR45148 before and after circular permutation is shown in supplemental Fig. S2.