TABLE 3.

Affinity and thermodynamic parameters of CtCBM62 binding to polysaccharides, oligosaccharides and monosaccharides

Ligand	Ka × 104	ΔG	ΔH	TΔS	na
	m−1	kcal mol−1	kcal mol−1	kcal mol−1
Galactomannan (Carob)	56.4 ± 3.1	−7.85 ± 0.03	−11.23 ± 0.06	−3.38 ± 0.03	1.00 ± 0.003
63,64-Di-α-d-galactosyl mannopentaose	2.21 ± 0.72	−4.56 ± 1.14	−8.69 ± 1.6	−4.13 ± 0.46	0.992 ± 0.15
61-α-d-Galactosyl mannotriose	0.29 ± 0.02	−4.33 ± 0.03	−7.04 ± 0.75	−2.71 ± 0.72	1.07 ± 0.89
Galactose	0.32 ± 0.003	−4.79 ± 0.01	−8.59 ± 0.17	−3.8 ± 0.16	1.07 ± 0.02
Mannopentaose	NBb
Xyloglucan (Tamarind)	177 ± 17.7	−8.53 ± 0.06	−11.63 ± 0.07	−3.1 ± 0.01	1.01 ± 0.003
XLXLG	2.01 ± 0.20	−5.87 ± 0.06	−10.74 ± 0.46	−4.87 ± 0.40	1.08 ± 0.036
XXXG	<0.01
Arabinogalactan (Larch)	84.1 ± 8.4	−8.09 ± 0.06	−13.16 ± 0.10	−5.07 ± 0.04	1.04 ± 0.005
Arabinan (debranched)	9.42 ± 0.49	−6.79 ± 0.03	−10.84 ± 0.10	−4.05 ± 0.07	1.02 ± 0.007
Arabinose	0.11 ± 0.005	−4.17 ± 0.02	−9.10 ± 0.20	−4.93 ± 0.18	0.99 ± 0.22
Xylan (oat spelt)	<0.01
Cellohexaose	NB
Cellotetraose	NB
Xylose	NB

^a The ITC data were fitted to a single site binding model for all ligands. For polysaccharide ligands, in which the molar concentration of binding sites is unknown, the n value was iteratively fitted as close as possible to one, by adjusting the molar concentration of the ligand.

^b NB, no binding.