FIGURE 2.
Analysis of the reaction products generated by CtXyl5A from arabinoxylan. Rye arabinoxylan was incubated with CtXyl5A until the reaction was complete, and the products were purified by size exclusion chromatography. Fraction 1 contained the most abundant oligosaccharides. A shows the MALDI-TOF MS analysis of permethylated and NaBH4-reduced oligosaccharides in fraction 1. Molecules that contained exclusively pentaose sugars are labeled Pen with the DP in subscript. B shows GC-EI-MS analysis of fraction 1. All the hydroxyls of T-Araf and T-Xylp are methylated, whereas 4-Xylp 3,4-Xylp, and 3-Xylp signify the positions of the hydroxyls that are not methylated and were thus involved in a linkage prior to TFA cleavage. C shows HPAEC analysis of fraction 1 treated with the arabinofuranosidase CjAbf51A. Peaks X2, X3, and X4 co-migrate with xylobiose, xylotriose, and xylotetraose. nc, nanocoulomb.