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. 2011 Mar 4;286(25):22510–22520. doi: 10.1074/jbc.M110.217315

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© 2011 by The American Society for Biochemistry and Molecular Biology, Inc.

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FIGURE 5. — Structure of the tetrasaccharides generated by CtXyl5A. Based on the data displayed in Fig. 4, the structures of the tetrasaccharides in fraction 1 were identified. The sugars labeled P can be Araf or Xylp. The data showed that the oligosaccharide ions colored green were present, and those colored red were not evident. The solid arrows between oligosaccharides show the conversion of one oligosaccharide into another, through ESI-MS fragmentation. Dotted arrows between oligosaccharides identify theoretical ESI-MS-mediated oligosaccharide conversions that did not occur in these analyses. The dotted arrow between sugar linkages within the oligosaccharides shows the fragmentation site and the ion identified. Arrows pointing at sugars (but do not link two sugars together) identify hydroxyl groups that were not methylated as they composed a glycosidic linkage in a parental ion. Xylol is the reducing end xylose that has been reduced to its alditol form by NaBH₄.