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The effect of phage T7 gp2 and phage T4 AsiA transcription initiation inhibitors on RNAP binding to downstream and upstream fork junction probes. The 8 nm [−12/+18][+3/+18] downstream fork junction shown in Fig. 1A or the 2 nm upstream fork junction shown in Fig. 2B was combined with RNAP beacon (1 nm) preincubated for 15 min with or without 0.2 μm T7 gp2 (A) or T4 AsiA (B) and increase in fluorescence was monitored.
