FIGURE 5.

The effect of junction point position on the strength of RNAP interaction with downstream fork junction probes. Time-dependent changes of the fluorescence signal were measured upon the addition of 50 μg/ml heparin to RNAP beacon complexes with fork junctions probes whose junction points were located between positions −2 to +6. The concentrations of RNAP beacon and fork junctions were 1 and 2 nm, respectively, except that concentration of the [−11/+26][−2/+26] probe was 4 nm.