Figure - PMC

Skip to main content

An official website of the United States government

Here's how you know

Here's how you know

Official websites use .gov
A .gov website belongs to an official government organization in the United States.

Secure .gov websites use HTTPS
A lock ( ) or https:// means you've safely connected to the .gov website. Share sensitive information only on official, secure websites.

View full-text article in PMC

. 2011 Apr 27;286(25):22609–22621. doi: 10.1074/jbc.M110.203885

Search in PMC
Search in PubMed
View in NLM Catalog
Add to search

© 2011 by The American Society for Biochemistry and Molecular Biology, Inc.

PMC Copyright notice

FIGURE 6. — PKCα is phosphorylated on serine 657 in response to AVP stimulation and is required for differentiation. A, the levels of phospho-Ser-657-PKCα were evaluated by Western blotting in L6 cells stimulated for different times by AVP. B, shown are the effects on AVP-induced PKCα phosphorylation of mTORC2 down-regulation induced by Rictor-siRNA (left panel) or 24 h of 100 nm rapamycin (rapa) treatment, or 40 min of treatment by mTOR inhibitor PP242 at 250 nm (right panel). C, phosphorylation of PKCα on Ser-657 was evaluated in cells overexpressing or not S6K1. D, the effects of siRNA-mediated PKCα depletion on myogenin protein expression were evaluated in AVP-stimulated L6 cells by Western blotting. The control of PKCα silencing efficiency is shown. *, significantly different from control cells, p < 0.05. NT, not transfected.