TABLE 2.
Mutational effects on ChVLig adenylylation
| Mutant | Preformed Lig–AMP in vivo (% of total enzyme) | Lig–[32P] AMP in vitro (% of total enzyme) |
|---|---|---|
| WT | 60 | 37 |
| F190A | 48 | 12 |
| S235A | 48 | 20 |
| T249A | 53 | 44 |
| K274A | 46 | 28 |
| K274Q | 27 | 45 |
| K274R | 35 | 46 |
| F276A/M278A | 60 | 31 |
| K281A/C283A | 36 | 41 |
| S235A/K281A/C283A | 75 | 22 |
| R285A | 45 | 34 |
| R285K | 18 | 32 |
| R285Q | 51 | 45 |
| F286A | 14 | 7 |
| F286L | 23 | 34 |
| V288A | 38 | 54 |
| V288I | 47 | 41 |
| V288T | 49 | 44 |
| N214A | 56 | 39 |
| N214D | 40 | 41 |
| N214L | 56 | 33 |
| N214Q | 53 | 36 |
| F215A | 53 | 39 |
| F215L | 60 | 40 |
| Y217A | 57 | 41 |
| Y217F | 57 | 41 |
| Y217L | 51 | 42 |
| Y217S | 56 | 42 |
| S218A/R220A | 37 | 44 |
| S221A/T222A/H223A | 58 | 39 |