TABLE 1.

Primers used for site-directed mutagenesis

The mutations are shown in boldface and underlined.

Mutation	Sequence (5′ to 3′)
I203M	Sense, GACTGCCACCATGATTGAGCCTT
	Antisense, TTAGACATACGAGAGCTGGACATAG
K309E	Sense, CAGGAAATTCCACAAGGATCGGT
	Antisense, AAAGGCTCAATGATGGTGGCAGTC
H211L	Sense, CTCAAAGATCGGTACTTGGCACAC
	Antisense, GAATTTCCTGAAAGGCTCAATGATGG
K212E	Sense, CACGAAGATCGGTACTTGGCACAC
	Antisense, GAATTTCCTGAAAGGCTCAATGATGG
R214G	Sense, CACAAAGATGGGTACTTGGCACAC
	Antisense, GAATTTCCTGAAAGGCTCAATGATGG
Y225F	Sense, GATCGGTACTTGGCACACCATAG
	Antisense, TTTGTGGAATTTCCTGAAAGGCT
F234S	Sense, GCAGCTAGCTCTGAACAAATGCA
	Antisense, AGCACTTCCACTGCTAGTATCCT
F234L	Sense, GCAGCTAGCTTGGAACAAATGCA
	Antisense, AGCACTTCCACTGCTAGTATCCT
E235K	Sense, GCAGCTAGCTTTAAACAAATGCA
	Antisense, AGCACTTCCACTGCTAGTATCCT
K239E	Sense, GAACAAATGCAGGAACAGAATAGA
	Antisense, AAAGCTAGCTGCAGCACTTCCACT
Q240H	Sense, AACATAATAGATTCCTTGGAAATG
	Antisense, TCTGCATTTGTTCAAAGCTAGCTG
R242G	Sense, AACAGAATGGATTCCTTGGAAATG
	Antisense, TCTGCATTTGTTCAAAGCTAGCTG
A201G/I203M	Sense, GACTGCCACCATGATTGAGCCTT
	Antisense, TTAGACATACGAGAGCTGGACATAG