TABLE 2.
Peripheral blood and vascular measurements in 25-week-old Ldlr−/− mice
Measurements are from nontransgenic mice (SR-uPA0/0) or littermate transgenic mice with macrophage-specific uPA overexpression (SR-uPA+/0) and were either wild type or null for the uPA receptor (Plaur+/+ or −/−). All mice were fed a high fat diet from 5–25 weeks of age. n = number of mice in each group. Data are the mean ± S.D. except for % coronary stenosis, which is ±S.E. p values are for comparison of nontransgenic and transgenic mice within each Plaur genotype and are from t tests except for peripheral blood monocytes, triglycerides, ORO-positive area, and coronary stenosis (Mann-Whitney tests).; IEL = internal elastic lamina.
|
Plaur+/+ |
Plaur−/− |
|||||||||
|---|---|---|---|---|---|---|---|---|---|---|
| SR-uPA0/0 | n | SR-uPA+/0 | n | P | SR-uPA0/0 | n | SR-uPA+/0 | n | P | |
| Peripheral blood monocytes (cells/mm3) | 83 ± 91 | 6 | 180 ± 158 | 5 | 0.2 | 81 ± 111 | 8 | 237 ± 201 | 6 | 0.08 |
| Monocytes (% of total leukocytes) | 2.0 ± 1.7 | 6 | 3.0 ± 1.4 | 5 | 0.3 | 2.1 ± 2.3 | 8 | 4.7 ± 3.8 | 6 | 0.3 |
| Plasma total cholesterol (mg/dl) | 1182 ± 154 | 16 | 1252 ± 328 | 8 | 0.5 | 1027 ± 203 | 16 | 1218 ± 220 | 13 | 0.02 |
| Plasma LDL cholesterol (mg/dl) | 189 ± 46 | 5 | 216 ± 36 | 3 | 0.4 | 195 ± 52 | 6 | 217 ± 48 | 5 | 0.5 |
| Plasma HDL cholesterol (mg/dl) | 89 ± 20 | 5 | 100 ± 8 | 3 | 0.4 | 103 ± 13 | 6 | 122 ± 24 | 5 | 0.1 |
| Plasma triglycerides (mg/dl) | 155 ± 86 | 16 | 144 ± 79 | 8 | 0.8 | 134 ± 89 | 16 | 127 ± 70 | 13 | 0.9 |
| Aortic root ORO-positive area (mm2) | 0.4 ± 0.1 | 11 | 0.8 ± 0.2 | 7 | <0.001 | 0.3 ± 0.1 | 12 | 0.7 ± 0.1 | 11 | <0.001 |
| ORO area (% intimal area) | 47 ± 5 | 11 | 46 ± 8 | 7 | 0.8 | 47 ± 7 | 12 | 46 ± 5 | 11 | 0.9 |
| Aortic root macrophage area (mm2) | 0.2 ± 0.06 | 9 | 0.5 ± 0.2 | 7 | <0.004 | 0.2 ± 0.06 | 10 | 0.4 ± 0.1 | 10 | <0.001 |
| Macrophage area (% intimal area) | 30 ± 8 | 9 | 32 ± 13 | 7 | 0.7 | 32 ± 7 | 10 | 29 ± 7 | 10 | 0.3 |
| Aortic total surface area (mm2) | 0.8 ± 0.07 | 15 | 0.78 ± 0.05 | 8 | 0.6 | 0.79 ± 0.07 | 11 | 0.76 ± 0.06 | 11 | 0.6 |
| IEL circumference (mm) | 5.6 ± 0.3 | 12 | 7.4 ± 0.5 | 8 | <0.001 | 5.3 ± 0.4 | 12 | 7 ± 0.3 | 6 | <0.001 |
| Maximal proximal coronary stenosis (%) | 47.3 ± 8.0 | 17 | 75.0 ± 7.4 | 20 | 0.05 | 17.5 ± 7.8 | 18 | 58.8 ± 8.0 | 17 | 0.002 |