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. 2011 May 2;286(25):22699–22705. doi: 10.1074/jbc.M111.237024

FIGURE 5.

FIGURE 5.

RTEF-1 regulates angiogenesis and blood flow recovery after ischemia in vivo. A, diagram of VE-cadherin promoter-driven human RTEF-1 transgene. B, immunoblot for RTEF-1 expression in the heart and aorta of endothelial cell-specific RTEF-1 transgenic mice (RTEF-1/TG). C, Western blot analysis of HIF-1α activation in RTEF-1/TG mice after intravenous injection of deferoxamine (DFX) to stabilize HIF-1α. D, RTEF-1/TG and wild-type mice were subjected to femoral artery ligation. Blood perfusion in ischemic hind limbs was measured by laser Doppler analysis on intermittent days until day 30. Sample images (upper panel) and quantification (lower panel) showing blood flow recovery at time points following ligation. *, significant increase of blood flow in the TG group when compared with the WT group from day 9 to day 21 (n = 5 for each group). E, sections of quadriceps from WT and RTEF-1/TG mice were stained for CD31 7 days after femoral artery ligation (n = 5; means ± S.D.). Top panel, images; bottom panel, quantification. *, p < 0.05.