Figure - PMC

Skip to main content

An official website of the United States government

Here's how you know

Here's how you know

Official websites use .gov
A .gov website belongs to an official government organization in the United States.

Secure .gov websites use HTTPS
A lock ( ) or https:// means you've safely connected to the .gov website. Share sensitive information only on official, secure websites.

View full-text article in PMC

. 2011 Jun 20;60(7):1901–1906. doi: 10.2337/db11-0120

Search in PMC
Search in PubMed
View in NLM Catalog
Add to search

© 2011 by the American Diabetes Association.

Readers may use this article as long as the work is properly cited, the use is educational and not for profit, and the work is not altered. See http://creativecommons.org/licenses/by-nc-nd/3.0/ for details.

PMC Copyright notice

FIG. 3. — Overexpressing glial GLUT1 in the hypothalamus of STZ-induced rats acutely normalizes plasma glucose levels. A: C6 (glial) and PC12 (neuronal) cells were transduced with adenovirus expressing GLUT1 with a GFAP promoter (Ad-GFAP-GLUT1). GLUT1 expression was measured 48-h post-transduction via immunoblotting. Significant GLUT1 overexpression was detected in C6 (*P < 0.05) but not in PC12 cells relative to control cell (CON). B: Hypothalamic tissues were obtained from rats injected with adenovirus expressing LacZ with a GFAP promoter (Ad-GFAP-LacZ) or Ad-GFAP-GLUT1 for 4 days. GLUT1 expression showed a 3.5-fold increase in GLUT1 expression in rat brains injected with GLUT1 (n = 4) adenovirus vs. LacZ (n = 4) adenoviral injection. *P < 0.05. C: Rat hypothalamic frozen sections obtained 4 days after the injection of Ad-GFAP-GLUT1. Sections were stained with anti-GLUT1, anti-GFAP, and anti-NeuN antibodies. Coimmunostaining indicated that the GLUT1 signals colocalized with GFAP, but not NeuN staining, in the arcuate nucleus (ARC). D: Plasma glucose levels in STZ-injected rats harboring Ad-GFAP-LacZ or Ad-GFAP-GLUT1. *P < 0.01 vs. MBH control Ad-GFAP-LacZ–injected rats. (A high-quality color representation of this figure is available in the online issue.)