FIG. 3.

FXR deficiency improves adipose tissue, but not hepatic insulin sensitivity in ob/ob mice. A: Female FXR^+/+ob/ob (13-week-old; white bars) and FXR^−/−ob/ob mice (black bars) (n = 4/group) were injected with either saline or insulin. Phosphorylation of Akt at Serine473 and total Akt protein expression was determined by Western blot and the signal quantified. B: Gene expression was measured by QPCR. C: Triglyceride content was determined enzymatically in livers of 20-week-old mice (n = 9 to 10/group). Values are means ± SEM. Differences between genotypes were calculated by Mann-Whitney test (*P < 0.05, **P < 0.01, ***P < 0.001). WAT, white adipose tissue; pAkt, Serine473-phosphorylated Akt; TG, triglyceride.