FIGURE 11.

Chemical cross-linking of cysteine residues at single sites on cdb3 and at single sites on AnkD34. The models in Fig. 10 predict residues that are at the protein-protein interface of the complex. These predictions were tested using the short cross-linking reagent BMOE. The SDS-PAGE results confirm that residues 160 and 254 on cbd3 are in close proximity to residues 598 and 631 on AnkD34. However, residues 160 and 254 on cdb3 do not form cross-links with residue 613 in the linker region of AnkD34 or with residues 616, 619, or 623 on the back convex surface.