FIGURE 4.

Representative EPR spectra from spin-labeled side chains incorporated into the α2 helix (126, 127, and 130), the α3 helix (152), and the β6/7 hairpin loop (179, 180, and 183) in the peripheral domain of cdb3. The spectra in solid lines were recorded in the absence of wt-AnkD34. The superimposed spectra in dashed lines were recorded after complex formation with wt-AnkD34. Also shown are the data from sites 160 and 254, which lie outside these three structured domains but which showed significant changes upon binding of wt-AnkD34. There were no changes in EPR line shapes upon complex formation at sites 133, 134, 137, 140, and 141 in the α1 helix, at sites 148 and 151 in the α2 helix, and at site 181 in the β6/7 hairpin loop (data not shown). 25 G, 25 Gauss.