FIGURE 5.
Changes in spin label side chain mobility (upper panel) and solvent accessibility (lower panel) as assessed by collision frequency with NiEDDA mapped onto the crystal structure of cdb3. The red residues in the upper panel showed a decrease in side chain mobility upon complex formation, the black residues showed no change, and the blue residues showed significantly impaired complex formation when the wt residue was changed to cysteine and spin-labeled with MTSSL. The same color scheme is used for the NiEDDA accessibility data in the lower panel for the black and blue residues, whereas the residues that showed changes in accessibility were displayed with three different colors based on their ΔΠ (Πcomplex-Πcdb3) values (red, ΔΠ < −15.0; hot pink, −15.0 ≤ ΔΠ < −5.0; pale pink, −5.0 ≤ ΔΠ < −2.0).