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. 2011 Apr 6;286(23):20536–20546. doi: 10.1074/jbc.M110.188326

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© 2011 by The American Society for Biochemistry and Molecular Biology, Inc.

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FIGURE 7. — Far Western blotting of human α5β1 integrin and recombinant NadA_24–210 protein. 1 μg of human α₅β₁ integrin was separated by SDS-PAGE and transferred onto nitrocellulose membranes. Integrins were renatured and detected with anti-β1 integrin antibody (lane 1), NadA specific antibody (lane 3), or Invasin specific antibody (lane 5) and peroxidase-conjugated secondary antibody. Additionally, renatured integrins were incubated with 5 μg of NadA_24–210 protein and detected with anti-NadA serum and peroxidase-conjugated secondary antibody (lane 2), or 5 μg of Inv397 (O:8) protein and detected with anti-Invasin serum and peroxidase-conjugated secondary antibody (lane 4). The arrow indicates the size of the β1 integrin subunit at 120 kDa.