TABLE 1.
Bacterial strains used in this study
| Strain | Description | Source or Ref. |
|---|---|---|
| Y. enterocolitica | ||
| WA-314 | clinical isolate of serotype O:8, carrying virulence plasmid pYVO8 | (42) |
| WA-314 ΔyadA | WA-314 harboring pYVO8-A-0 with yadA replaced by a kanamycin (kmR) resistance cassette | (23) |
| WA-314 ΔyadA:SS | WA-314 harboring pYVO8-SS | This study |
| WA-314 ΔyadA:yadA | WA-314 harboring pYVO8-A-1 with integrated pGPS-A-1, complemented by wild-type yadA | (23) |
| WA-314 ΔyadA:nadA | WA-314 harboring pYVO8-nadA | This study |
| WA-c | plasmidless derivate of WA-314 | (42) |
| WA-c Δinv | inv-negative mutant of WA-c | (43) |
| WA-c Δinv(p) | WA-c Δinv harboring plasmid p (pACYC184 with SalI/SphI fragment of virF) | This study |
| WA-c Δinv(pyadA) | WA-c Δinv harboring plasmid pyadA (plasmid p with XbaI/BamHI fragment of yadA) | This study |
| WA-c Δinv(pnadA) | WA-c Δinv harboring plasmid pnadA (plasmid p with XbaI/BamHI fragment of nadA) | This study |
| WA-c Δinv(pYVO8-SS) | WA-c Δinv harboring pYVO8-SS | This study |
| WA-c Δinv(pYVO8-nadA) | WA-c Δinv harboring pYVO8-nadA | This study |
| N. meningitides | ||
| MC58 | serogroup B, serotype 74, clinical isolate of the ST-32 complex | kindly provided by M. Frosch |
| MC58 ΔsiaD | MC58 with deletion of siaD | (44) |
| MC58 ΔsiaD ΔnadA | MC58 with deletion of siaD and nadA | This study |
| N. gonorrhoeae | ||
| N. gonorrhoeae Ngo OpaCEA | OpaCEA-expressing (Opa52), non-piliated N. gonorrhoeae MS11-B2.1, strain N309 | (45) |
| N. gonorrhoeae Ngo Opa- | Non-opaque, non-piliated N. gonorrhoeae MS11-B2.1, strain N302 | (45) |
| E. coli | ||
| DH5α | endA1 supE44 hsdR17 (rk− mk+) thi-1 recA1gyrA96 relA1 Δ(lacZYA-argF) U169 (ϕ80lacZΔM15) | (46) |
| SM10λpir | thi-1 thr leu tonA lacy supE recA:: RP4–2-TC:: Mu-Kan (λ pir), Kmr | (47) |
| BL21 (DE3) | protein expression strain; F–dcm ompT hsdS (rB– mB–) gal λ(DE3) | (48) |