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. 2011 Apr 19;286(23):20582–20590. doi: 10.1074/jbc.M111.237438

TABLE 1.

Comparison of selected terpene synthase enzyme kinetic parameters

Enzyme Product kcat Km Hill coefficient Ref.
s−1
Malus domestica farnesene synthase Farnesene 0.0553 10–25 μma 37, 47
M. domestica farnesene synthase Monoterpenes 0.00028 50 μma 37
Mentha spicata limonene synthase Limonene 0.024 6.7 μm 41
M. spicata 1,8-cineole synthase 1,8-Cineole 0.053 65 μm 48
P. alba isoprene synthase Isoprene 0.03 8.7 mm 11
Populus x canescens isoprene synthase Isoprene 0.26 2.45 mm 49
P. montana isoprene synthase Isoprene 0.088 7.7 mm 4.1 9
P. sabiniana MBO synthase MBO 0.015 20.0 mm 1.2 This study
P. sabiniana MBO synthase Isoprene 0.0004 18.2 mm 1.4 This study

a This is the concentration used in the assay when the Km was not reported.