FIGURE 1.

Extensive neuronal cell death occurs between 8 and 24 h after Aβ administration. A, shown is cell viability as measured by ATP content in hippocampal neuronal cultures treated with the indicated concentrations of Aβ as a function of time. 2.5 μm Aβ produces ∼50% cell death after 24 h of exposure. Error bars represent S.E. of three independent experiments. B, shown is cell viability as measured by LDH released into the media when hippocampal neurons are treated with 2.5 μm Aβ for the indicated times. Error bars represent S.E. of at least three independent experiments. C, shown is immunofluorescence microscopy images of hippocampal neurons treated with 2.5 μm Aβ for the indicated times. Anti-Tau (pan-Tau) is red, and anti-α-tubulin (DM1A) is green. Magnification, 20×; scale bar, 10 μm. *, p < 0.05; **, p < 0.01 compared with controls. UN, untreated.