FIGURE 5.

Rolling circle DNA synthesis by WT and A257T gp4 in the presence of T7 DNA polymerase. A, schematic shows the 70-bp minicircle replication fork. B, WT or A257T gp4 at 100 nm was incubated with 100 nm T7 DNA polymerase (T7 gp5-thioredoxin) and minicircle DNA (50 nm), and DNA synthesis was measured using [α-³²P]dGTP in the presence of all dNTPs. The DNA products are resolved by electrophoresis on a 1% alkaline-agarose gel. Images show a time course of increasing kilobase pair length of DNA synthesis by WT and A257T in presence of T7 DNA polymerase.