FIGURE 2.

Neuronal localization of Slc38a7. In situ hybridization combined with immunohistochemistry on 7-μm paraffin mouse brain sections for co-localization of Slc38a7 mRNA and known cellular markers. Co-expression with the glial cell marker GFAP are shown on the first row (A), the neuronal protein markers NeuN are shown on the second row (B), and GAD67 on the third row (C). The cell nuclei marker DAPI was stained in blue, the Slc38a7 mRNA in red, whereas other markers were labeled in green. The mRNA expression of Slc38a7 only shows overlap with the excitatory and inhibitory neuronal marker (×20 objective), and not with the glial cell marker (×40 objective). The thick arrows indicate cells expressing the marker and thin arrows Slc38a7 expressing cells.