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. 2011 Apr 25;286(23):20251–20259. doi: 10.1074/jbc.M110.211771

FIGURE 2.

FIGURE 2.

Interaction between ATF5 and HSP70. A, reciprocal immunoprecipitation (IP) analysis showing interaction between ATF5 and HSP70. The C6-pCIN4 and C6-pCIN4-FLAG-HA-ATF5 cell lines were used for cell extract preparation. The expression of endogenous HSP70 and FLAG-HA-ATF5 were detected using antibodies against HSP70 and HA as indicated (Input). Antibodies used for reciprocal IP and immunoblotting (IB) analyses for detection of associated proteins are as indicated. B, immunoprecipitation analysis showing that endogenous ATF5 interacts with HSP70 but not with HSP90. C6 cells were used for IP, and indicated antibodies were used in the immunoblotting analyses. B-Raf is a known HSP90 substrate protein and was used as a control. C, C6 cells transiently transfected with indicated constructs were used for IB and IP analyses as in A.