FIGURE 2.
Effect of amino acid substitutions S68G/Δ69/K70G and Δ67/T69G/K70R on rescue DNA polymerization reactions catalyzed by RTs carrying the thymidine analog mutations M41L and T215Y. Time courses of reactions initiated from primers terminated with AZTMP (A) and d4TMP (B), and carried out in the presence of 3.2 mm ATP (left) or 200 μm PPi (right). All dNTPs were supplied at 100 μm, except for dATP, whose concentration was 1 μm. The concentration of template/primer (D38/25PGA) was 30 nm. RTs were used at 72 nm and 24 nm concentrations (active sites), in rescue reactions carried out in the presence of ATP or PPi, respectively. Represented values were obtained from at least three independent experiments. Rescue rates (kobs values) are given in the supplemental Table S2.