FIGURE 5.
Effects of amino acid substitution K219E on rescue DNA polymerization reactions catalyzed by RTs carrying deletions in the β3-β4 hairpin loop. Time courses of reactions initiated from primers terminated with AZTMP (A) and d4TMP (B), and carried out in the presence of 3.2 mm ATP (left) or 200 μm PPi (right). All dNTPs were supplied at 100 μm, except for dATP, whose concentration was 1 μm. The concentration of the template/primer (D38/25PGA) was 30 nm. RTs were used at 72 and 24 nm concentrations (active sites), in rescue reactions carried out in the presence of ATP or PPi, respectively. Represented values were obtained from at least three independent experiments. Rescue rates (kobs values) are given in the supplemental Table S2.