FIGURE 6.

Effect of amino acid substitution K219E on GTP-dependent excision efficiencies with primers terminated with AZTMP. Rescue DNA polymerization assays were carried out in the presence of 9.6 mm GTP. All dNTPs were supplied at 100 μm, except for dATP, whose concentration was 1 μm. Template primer (D38/25PGA) and active RT concentrations in these assays were 30 nm and 72 nm, respectively. The lower panels show direct comparisons between rescue efficiencies of RTs with and without K219E, at selected times. Represented values were obtained from at least three independent experiments.