FIGURE 2.

Gel filtration elution profiles of wild type BlcR and BlcR^F147A in the absence and presence of SSA. The protein was incubated with SSA in a molar ratio of 1:10 (monomeric BlcR/SSA), and 150 μm SSA was included in the mobile phase (50 mm Tris-Cl, pH 7.5, 300 mm NaCl, and 0.5 mm EDTA) of a Superdex200 column. Solid line, BlcR without SSA; dashed line, BlcR with SSA; dashed and dotted line, BlcR^F147A without SSA; dotted line, BlcR^F147A with SSA. Proteins used for molecular weight calibration are shown at their elution volumes.