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Effect of mutations on binding of BlcR with SSA
| Proteins |
Tma |
ITC Kd | |||
|---|---|---|---|---|---|
| 1:0b | 1:1 | 1:5 | 1:10 | ||
| °C | μm | ||||
| BlcR | 54.5 | 57 | 58.4 | 60 | 0.7 ± 0.1 |
| BlcRY133A | 44.0 | 44.5 | 49.5 | 49.5 | 19.3 ± 3.1 |
| BlcRF147A | 45.5 | 46.5 | 49 | 50 | 3.9 ± 0.3 |
| BlcRT158A | 49.5 | 53.5 | 56 | 56.5 | 1.6 ± 0.3 |
| BlcRD210A | 48.0 | 48.0 | 48.0 | 48.0 | Weakc |
| BlcRC220A | 47.0 | 47.0 | 47.0 | 47.0 | NDd |
a Melting temperatures are from DSF studies.
b BlcR monomer/SSA ratio.
c Heat exchange was too low for accurate ITC analysis.
d ND, not detected.
