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. 2011 Apr 22;286(23):20991–21001. doi: 10.1074/jbc.M111.226266

FIGURE 5.

FIGURE 5.

Effect of subretinal administration of anti-murine CCL2 on MAC deposition, VEGF expression, and formation of CNV complex. Representative confocal micrographs of RPE-choroid-sclera flat mounts immunostained for MAC at 12 h (A) and day 3 (C) post-laser treatment from mice injected with anti-murine CCL2 or isotype control antibody via subretinal route. Graphs show semi-quantitative evaluation of positive fluorescent signal for MAC at 12 h (B) and at day 3 (D). VEGF mRNA (E) and protein (F) were determined at day 3 post-laser treatment by real-time RT-PCR and ELISA, respectively, in RPE-choroid. Representative confocal microphotographs of RPE-choroid-sclera flat mounts from C57BL/6 mice injected sub-retinally with anti-murine CCL2 or isotype control antibody and sacrificed at day 7 post-laser treatment are shown (G). Cumulative data obtained by the quantification of the images are shown (H). Quantification and statistical analyses, including S.D. and Student's t test, were performed as described under “Experimental Procedures.” All data are representative of three independent experiments. *, p < 0.05.