Skip to main content
. 2011 Apr 22;286(23):20991–21001. doi: 10.1074/jbc.M111.226266

FIGURE 6.

FIGURE 6.

Effect of i.p. injection of anti-murine VEGF on MAC deposition, CCL2 expression, and formation of CNV complex. A and C show representative confocal micrographs of RPE-choroid-sclera flat mounts immunostained for MAC at 12 h and day 3 post-laser treatment, respectively, from mice injected i.p. with anti-murine VEGF or control antibody. Semi-quantitative evaluation of positive fluorescent signal for MAC at 12 h (B) and at day 3 (D) is shown. CCL2 mRNA (E) and protein (F) were analyzed at 12 h post-laser treatment by real-time RT-PCR and ELISA, respectively, in RPE-choroid of these animals. Representative confocal microphotographs of RPE-choroid-sclera flat mounts from C57BL/6 mice injected with anti-murine VEGF or control antibody and sacrificed at day 7 post-laser treatment are shown (G). H represents the cumulative data of CNV quantification. Quantification and statistical analyses, including S.D. and Student's t test, were performed as described under “Experimental Procedures.” All data are representative of three independent experiments. *, p < 0.05.