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. 2011 Apr 20;286(23):20942–20951. doi: 10.1074/jbc.M111.234641

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© 2011 by The American Society for Biochemistry and Molecular Biology, Inc.

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FIGURE 7. — Gephyrin interacts with NLG2 and NLG1. A, GST-NLG1/2_CD pulldown assay using lysates of HEK-293 cells transfected with gephyrin-FLAG (left) and rat brain lysates (right). B, lysates of HEK-293 cells transfected with either NLG2-HA (left) or NLG1-HA (right) in the presence of gephyrin-FLAG or with the vector alone (as a negative control) immunoprecipitated with monoclonal anti-FLAG antibodies. Immunoprecipitates were analyzed by Western blotting using anti-HA and anti-FLAG monoclonal antibodies. C, co-immunoprecipitation experiments on rat brain lysates using a monoclonal anti-gephyrin antibody and normal mouse serum (NMS) as negative control. Immunoprecipitates were analyzed by Western blotting using a monoclonal anti-gephyrin antibody and a polyclonal antibody against NLG2 and NLG1.