FIGURE 9.

Co-sedimentation of actin, tropomyosin, and troponin complex containing either WT or mutant TnT. The experimental conditions were outlined under “Experimental Procedures.” Samples were separated by 15% SDS-PAGE. S, supernatant; P, pellet. A, lanes 1 and 5, WT troponin complex supernatant (pellet not shown); lanes 2 and 6, ΔN100/ΔE101troponin complex supernatant (pellet not shown); lanes 3 and 7, actin-tropomyosin supernatant; lanes 4 and 8, actin-tropomyosin pellet. B, lanes 1 and 5, actin-tropomyosin-WT troponin supernatant; lanes 2 and 6, actin-tropomyosin-WT troponin pellet; lanes 3 and 7, actin-tropomyosin-ΔN100/ΔE101 troponin supernatant; lanes 4 and 8, actin-tropomyosin-ΔN100/ΔE101 troponin pellet. Lanes 1–4 were performed at a low Ca²⁺ concentration. Lanes 5–8 were performed at a high Ca²⁺ concentration.