TABLE 1.
Summary of SR purification
Sulfur reductase was purified as described under “Experimental Procedures.” Purification steps monitored by GR activities are shown in parentheses.
| Purification procedure | Protein | Total activity | Specific activity | Yield | Purification |
|---|---|---|---|---|---|
| mg | μmol min−1 | μmol min−1mg−1 | % | Fold | |
| Cell-free extract | 750 | 180 (250) | 0.24 (0.37) | 100 (100) | 1.0 (1.0) |
| Ultracentrifugation | 400 | 140 (200) | 0.35 (0.51) | 78 (75) | 1.4 (1.4) |
| Butyl-Sepharose | 22 | 66 (110) | 3.0 (4.8) | 37 (37) | 11 (13) |
| 2′5′-ADP-Sepharose | 0.74 | 29 (44) | 41 (59) | 16 (18) | 150 (160) |