FIGURE 2.

ATP synthase in the mitochondria in shMock and DAPIT-knockdown cells. Mitochondria (5 μg of protein) were analyzed with Clear Native PAGE (A) or SDS-PAGE (B). The protein bands of α-subunit, β-subunit, and DAPIT were stained with Western blotting. α-Subunit was stained strongly to show the presence of free subunit. The positions of ATP synthase, free α-subunit, and free β-subunit in A are indicated. Experiments were carried out at least five times, and representative results are shown.