FIGURE 4.

Mitochondrial activities of ATP synthesis (A) and membrane potential generation (B) of shMock and DAPIT-knockdown cells. A, ATP synthesis activities were measured with luciferase (16). Filled symbols represent the data in the presence of oligomycin (10 μg/ml) of the corresponding open symbols. B, −CCCP, fluorescent images of cells incubated for 10 min with TMRE that accumulates in mitochondria in response to establishment of membrane potential. +CCCP, fluorescent images of the same field after addition of an uncoupler CCCP that dissipates membrane potential and proton gradient. Details are described under “Experimental Procedures.”