FIGURE 4.

MSC dynamically localizes to VV factories. HeLa cells infected with VV for 4 h were fixed (A) or Dig extracted prior to fixation (B) and staining with the anti-ARS Abs indicated. Cytosolic Hoechst 3358 staining identifies viral DNA in factories (arrowheads). Bar scale, 10 μm. C, to obtain larger factories, cells were infected for 7 h with VV, and then pulsed with puromycin, extracted with Dig prior to fixation and stained for puromycin and the anti-ARS Ab indicated. Bar scale, 10 μm. C and D, E, after 7 h VV infection, cells were extracted or not with Dig prior to fixation and stained with Abs specific for SRS or eIF3η. Bar scale, 10 μm.