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. 2011 Feb 22;286(23):20228–20238. doi: 10.1074/jbc.M110.209528

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© 2011 by The American Society for Biochemistry and Molecular Biology, Inc.

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FIGURE 4. — Visualization of the mineralization process in NIH 3T3-PP cells. Panel A shows a von Kossa stain of control NIH 3T3 cells (day 10); negligible staining indicates a lack of mineralization. Panel B shows a positive von Kossa stain at day 10 of transfected NIH 3T3-PP cells indicating the presence of mineralization. Panels C–F, cells stained at day 10 with von Kossa and counterstained with fuchsin red. Panels C and D, non-transfected cells (control) shown at ×10 and 20 magnification, respectively (scale bar = 200 μm). Panels E and F show transfected cells with multiple mineral deposit foci at ×10 and 20 magnification, respectively.