TABLE 1.

Catalytic activity of metal-substituted MshB

Apo-MshB was incubated with stoichiometric metal for 45 min prior to activity measurement. The substrate used was GlcNAc.

	Km	kcat	kcat/Km
	mm	s−1	m−1s−1
Apo-MshB	36 ± 7	0.13 ± 0.01	3.5 ± 0.3
Co2+-MshB	34 ± 5	1.1 ± 0.08	32 ± 2
Fe2+-MshB	41 ± 5	2.1 ± 0.15	52 ± 3
Fe3+-MshB	56 ± 13	0.11 ± 0.01	1.8 ± 0.3
Mn2+-MshB	54 ± 13	0.38 ± 0.04	7.0 ± 1.0
Ni2+-MshB	53 ± 14	0.27 ± 0.03	5.3 ± 0.8
Zn2-MshBa	38 ± 4	0.77 ± 0.04	20 ± 1

^a Data were adapted from Ref. 32. For comparison, substrate = GlcNAc-Ins, K_m = 340 ± 80 μm, k_cat = 0.49 ± 0.04 s⁻¹, k_cat/K_m = 1440 ± 360 m⁻¹ s⁻¹ as reported previously (16).