FIGURE 2.

NMDA elicits significant neuronal death in hippocampus of adult and young rats but not of embryonic and newborn rats. Rats (n = 6) of indicated age were subjected to i.c.v. injection of NMDA (60 mm, 1 μl) in one side and PBS in the other side and killed after 24 h. Sections were Nissl-stained. Representative images showing the loss of Nissl stain in hippocampus of adult (3 month) and PND12 rats but not in those of E18 and P0 rats, n = 3. Boxed regions are amplified and inserted at the bottom left or bottom right. Bar, 100 μm.