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. 2011 Apr 20;286(23):20606–20614. doi: 10.1074/jbc.M110.216549

FIGURE 3.

FIGURE 3.

Role of the 24p3/24p3R pathway in stress hematopoiesis. A, bone marrow cells were harvested from both hind limbs of either 24p3−/− or control WT mice (both on C57BL/6 background CD45.2 +) and mixed with competitor Ly-5.1 BoyJ bone marrow cells (CD45.2+) at a ratio of 1:1. The mixed bone marrow cells were injected into lethally irradiated (11 Gy (1100 rads)) Ly-5.1 BoyJ recipient mice at a ratio of 1 donor equivalent to five recipients. The percentage of donor-derived CD45.2 chimerism in the peripheral blood of each recipient mouse was determined by flow cytometry. The donor engraftment was analyzed 9 weeks or later after the transplantation. Results shown are mean ± S.D. (error bars) from two independent transplantations with a total of 10 mice in each group. B, percentage of donor-derived chimerism in Gr-1- and B220-positive populations in the peripheral blood of each recipient mouse. The donor engraftment was analyzed 6 months post-transplantation. Results shown are mean ± S.D. from 24p3+/+ (n = 7) and 24p3−/− (n = 8). C and D, 5-FU was injected intraperitoneally (200 mg/kg), and at 0 days (before injection) and 7 days (after injection), mice were euthanized, and bone marrow from hind limbs (femur and tibia) was collected. The bone marrow counts are shown for total white blood cells (C) and for the Sca-1+Lin− fraction (D).