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. 2011 Apr 18;286(23):20913–20922. doi: 10.1074/jbc.M110.213678

FIGURE 7.

FIGURE 7.

Semiquantitative RT-PCR analyses of CgHXT1 to CgHXT5 expression levels. A, expression levels of the Histone3 gene under the same conditions is shown as control. RT-PCRs were performed on total RNA isolated from axenically grown C. graminicola grown for 3 days at 23 °C in the presence of the indicated additions (glucose, 1.5%; extract, maize cell walls plus soluble extract from maize leaves as described under “Experimental Procedures.” One of three biological repeats is shown. Transcript levels of the histone H3 gene under the same conditions is shown as control. B, transcript abundances of CgHXT1 to CgHXT5 were studied in C. graminicola-infected maize leaves at 2, 3, or 4 dpi. mRNA levels of the C. graminicola actin and histone H3 genes under the same conditions are shown as controls. RT-PCRs were performed on total RNA isolated from uninfected or infected maize leaves (maize plus fungal RNA) at the indicated times (one of three biological repeats is shown).