FIGURE 8.

Quantitative RT-PCR analyses of CgHXT1 to CgHXT5 transcript levels in axenically grown C. graminicola. mRNA levels of CgHXT1 to CgHXT5 were studied in C. graminicola grown for 3 days at 23 °C in the presence of the indicated additions (glucose, 1.5%; extract, maize cell walls plus soluble extract from maize leaves as described under “Experimental Procedures.”). mRNA levels in C. graminicola cells incubated without glucose and extract (dark bars) were set to 100%. Histone3 transcript levels served as controls. The RNA were obtained from 3 biological repeats (± S.E.) and were different from those shown in Fig. 7A.