TABLE 1.
Primers used for the amplification of CgHXT1 to CgHXT5 cDNAs or for the quantification of mRNA abundance by semiquantitative or quantitative RT-PCR
Gene | Primer name | Sequence |
---|---|---|
cDNA amplification: | ||
CgHXT1 | CgHXT1(#13)fw | 5′-CTCTCTGAATTCCCCCTTCGTCATCATGGGTA-3′ |
CgHXT1(#13)rev | 5′-GAGAGAGAATTCCACACGCAATAAACAG | |
CAGACA-3′ | ||
CgHXT2 | CgHXT2(#29)fw1 | 5′-CTCTCTGAATTCATGGGGTTGGGCAAACTGTA-3′ |
CgHXT2(#29)rev | 5′-GAGAGAGAATTCCCCTGTCTCTTATACAA | |
CATTCATACG-3′ | ||
CgHXT3 | CgHXT3(#27)fw | 5′-CTCTCTGAATTCAAACCACAACAGCCCTCCAC-3′ |
CgHXT3(#27)rev | 5′-GAGAGAGAATTCCGAGACGTCGGCTTAAACAT-3′ | |
CgHXT4 | CgHXT4(III)fw | 5′-CTCTCTGAATTCCCCACAGTCCCATTCACCAT-3′ |
CgHXT4(III)rev | 5′-GAGAGAGAATTCAGTGGCAATGGTAAC | |
CAGCA-3′ | ||
CgHXT4-pEX-GFPr | 5′-TCTCTGAATTCCATAGCTGAAGTCCACATTGCC-3′ | |
CgHXT5 | CgHXT5(IV)fw | 5′-CTCTCTGAATTCGATACCCCCGTCAACAGC-3′ |
CgHXT5rev#3 | 5′-GAGAGAGAATTCTTAGACGGTGTTTTCA | |
GCCTTC-3′ | ||
Quantification by RT-PCR: | ||
CgACT | CgACT-qRT.F1 | 5′-TTCTACGAGCTTCCTGACGG-3′ |
CgACT-qRT.R1 | 5′-CCGCTCTCAAGACCAAGGAC-3′ | |
CgHXT1 | CgHXT1(#13)fw | 5′-ATCGCCTTCGTCTACTTCTTCAT-3′ |
CgHXT1(#13)rev | 5′-CTAGGCAGAGGCGGTCTTCT-3′ | |
CgHXT2 | UL13(fw) | 5′-TTGTCTCTGCCTGCATGTTC-3′ |
UL14(rev) | 5′-AGTTGCGTAGGGAGTGAGGA-3′ | |
CgHXT3 | UL19(fw) | 5′-ATTGTCGCTGGTCGTCTCAT-3′ |
UL20(rev) | 5′-CAATGGTGATGCAGAACTGG-3′ | |
CgHXT4 | UL1(fw) | 5′-ATTCTTCTTCGCAGCATC-3′ |
UL2(rev) | 5′-AAGACCATGCACCACACAAA-3′ | |
CgHXT5 | UL3(fw) | 5′-GACACCAGCATCTGGGAAAT-3′ |
UL4(rev) | 5′-GGTGACGTAGCTGTTGCTGA-3′ |