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. 2011 Apr 18;286(23):20913–20922. doi: 10.1074/jbc.M110.213678

TABLE 1.

Primers used for the amplification of CgHXT1 to CgHXT5 cDNAs or for the quantification of mRNA abundance by semiquantitative or quantitative RT-PCR

Gene Primer name Sequence
cDNA amplification:
    CgHXT1 CgHXT1(#13)fw 5′-CTCTCTGAATTCCCCCTTCGTCATCATGGGTA-3′
CgHXT1(#13)rev 5′-GAGAGAGAATTCCACACGCAATAAACAG
CAGACA-3′
    CgHXT2 CgHXT2(#29)fw1 5′-CTCTCTGAATTCATGGGGTTGGGCAAACTGTA-3′
CgHXT2(#29)rev 5′-GAGAGAGAATTCCCCTGTCTCTTATACAA
CATTCATACG-3′
    CgHXT3 CgHXT3(#27)fw 5′-CTCTCTGAATTCAAACCACAACAGCCCTCCAC-3′
CgHXT3(#27)rev 5′-GAGAGAGAATTCCGAGACGTCGGCTTAAACAT-3′
    CgHXT4 CgHXT4(III)fw 5′-CTCTCTGAATTCCCCACAGTCCCATTCACCAT-3′
CgHXT4(III)rev 5′-GAGAGAGAATTCAGTGGCAATGGTAAC
CAGCA-3′
CgHXT4-pEX-GFPr 5′-TCTCTGAATTCCATAGCTGAAGTCCACATTGCC-3′
    CgHXT5 CgHXT5(IV)fw 5′-CTCTCTGAATTCGATACCCCCGTCAACAGC-3′
CgHXT5rev#3 5′-GAGAGAGAATTCTTAGACGGTGTTTTCA
GCCTTC-3′
Quantification by RT-PCR:
    CgACT CgACT-qRT.F1 5′-TTCTACGAGCTTCCTGACGG-3′
CgACT-qRT.R1 5′-CCGCTCTCAAGACCAAGGAC-3′
    CgHXT1 CgHXT1(#13)fw 5′-ATCGCCTTCGTCTACTTCTTCAT-3′
CgHXT1(#13)rev 5′-CTAGGCAGAGGCGGTCTTCT-3′
    CgHXT2 UL13(fw) 5′-TTGTCTCTGCCTGCATGTTC-3′
UL14(rev) 5′-AGTTGCGTAGGGAGTGAGGA-3′
    CgHXT3 UL19(fw) 5′-ATTGTCGCTGGTCGTCTCAT-3′
UL20(rev) 5′-CAATGGTGATGCAGAACTGG-3′
    CgHXT4 UL1(fw) 5′-ATTCTTCTTCGCAGCATC-3′
UL2(rev) 5′-AAGACCATGCACCACACAAA-3′
    CgHXT5 UL3(fw) 5′-GACACCAGCATCTGGGAAAT-3′
UL4(rev) 5′-GGTGACGTAGCTGTTGCTGA-3′