TABLE 2.
Proteins in rat spinal cord extracts interacting with immobilized apoG93A hSOD1
Recombinant apo G93A mutant hSOD1 were immobilized on CNBr-Sepharose. The gels were incubated with rat spinal cord extracts and protein binding to hSOD1 were isolated, separated by two-dimensional gel electrophoresis and identified with MALDI-TOF-MS.
| Protein | Abbreviation | Accession no. (UniProtKB) | Sequence coverage (%) | Score/no. of peptides |
|---|---|---|---|---|
| Heat shock cognate 70 kDa protein | Hsc70 | P63018 | 24 | 143/14 |
| Glyceraldehyde-3-phosphate dehydrogenase | GAPDH | P04797 | 50 | 160/12 |
| β-Actin | P60711 | 40 | 163/12 | |
| Tubulin β2B | P04691 | 42 | 172/24 | |
| Tubulin α1a | P68370 | 40 | 170/13 | |
| 78 kDa glucose-regulated protein | Grp78 | P06761 | 33 | 161/18 |
| Copper chaperone for superoxide dismutase | CCS | Q9JK72 | 33 | 172/12 |
| Glutamine synthetase | GLNA | P09606 | 51 | 269/23 |
| ATP synthase subunit β | ATPB | P10719 | 22 | 68/10 |
| Heat shock 27 kDa protein | Hsp27 | P42930 | 37 | 126/8 |
| Ribonuclease inhibitor | RINI | P29315 | 32 | 104/10 |
| ATP synthase subunit α | ATPA | P15999 | 28 | 143/16 |
| αβ-crystallin | P23928 | 49 | 79/10 | |
| Neurofilament light polypeptide | NF-L | P19527 | 46 | 204/25 |