Fig. 2.

Testis development in the Dkk1-null embryos appears normal with respect to expression of marker genes. A qRT-PCR analysis of transcriptional targets of the canonical WNT pathway, B markers of ovarian development, and C markers of testicular development in 12.5 dpc UGR samples from wild-type (Wt), heterozygous (het) and null (KO) Dkk1 embryos. Bars indicate the mean ± 1 SEM; n = 5, 3, 5, 2, 4, 3. Endogenous control Tbp (encoding TATA box binding protein) was used to normalize gene expression levels.