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. Author manuscript; available in PMC: 2011 Jun 23.
Published in final edited form as: Pharm Res. 2008 Nov 11;26(3):523–528. doi: 10.1007/s11095-008-9767-0

Fig. 2.

Fig. 2

High-level expression by the insertion of helical linker in both hGH-Tf and Tf-hGH fusion proteins as analyzed by Anti-Tf Western blot. Four fusion proteins with or without the inserted helical linker, expressed in serum free media, were analyzed by Western blot using goat anti-Tf antibody (1:5000). The signal was detected using rabbit anti-goat secondary antibody conjugated to HRP (1:1000) and ECL reagents. The image was recorded and analyzed by ChemiDoc XBR. Lane 1: Tf (50 ng); lane 2: hGH (negative control); lane 3: hGH-Tf; lane 4: hGH-(H4)2-Tf; lane 5: Tf-hGH; lane 6: Tf-(H4)2-hGH.