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The ratio of expression of Tf-fusion proteins with or without helical linker
| Linker (x) | G-CSF Fusion Proteins | hGH Fusion Proteins | ||
|---|---|---|---|---|
| Tf-x-G-CSF | G-CSF-x-Tf | Tf-x-hGH | hGH-x-Tf | |
| No Linker | 1 | 1 | 1 | 1 |
| H4 | 7.8 | ND | ND | ND |
| (H4)2 | 11.2 | 1.44 | 2.39 | 1.66 |
x: linker inserted between Tf and hGH or G-CSF.
(H4): helical linker.
(H4)2: two copies of helical linker.
no linker: two domains were fused by LE peptide
ND: not determined.
